Toxoplasma gondii test for men

Many women will undergo Toxoplasma examination before pregnancy and during a specific period of pregnancy, especially families that previously raised cats and dogs. Of course, in order to give birth to a healthier baby, men also need to undergo a Toxoplasma test during pregnancy preparation. This can ensure that both couples have no problems and can conceive normally. So, how do men get checked for Toxoplasma gondii? This will be introduced below!

1. Direct microscopic examination of the patient's blood, bone marrow or cerebrospinal fluid, thoracic and ascites fluid, sputum, bronchoalveolar lavage fluid, aqueous humor, amniotic fluid, etc. for smears, or biopsies of lymph nodes, muscles, liver, placenta, etc., for Wright's or Gibbon staining microscopy, trophozoites or cysts can be found, but the positive rate is not high. Direct immunofluorescence can also be used to detect Toxoplasma gondii in tissues.

2. Animal inoculation or tissue culture: Take the body fluid or tissue suspension to be tested and inoculate it into the abdominal cavity of mice. Infection can occur and pathogens can be found. If the first generation of inoculation is negative, it should be blindly passaged 3 times. Or tissue (monkey kidney or pig kidney cells) culture can be used to isolate and identify Toxoplasma gondii.

3. DNA hybridization technology. For the first time, domestic scholars used 32P-labeled probes containing specific DNA sequences of Toxoplasma gondii to conduct molecular hybridization with the DNA of cells or tissues in the patient's peripheral blood. The specific hybridization bands or spots showed a positive reaction. Both specificity and sensitivity are high. In addition, polymerase chain reaction has been established in China to diagnose this disease, and compared with probe hybridization, animal vaccination and immunological examination methods, it has been shown to be highly specific, sensitive and rapid.

1. The antigens used to detect antibodies mainly include tachyzoite soluble antigens (cytoplasmic antigens) and membrane antigens. Antibodies in the former appear earlier (detected by staining test, indirect immunofluorescence test), while antibodies in the latter appear later (detected by indirect hemagglutination test, etc.). Using multiple detection methods at the same time can play a complementary role and increase the detection rate. Since Toxoplasma gondii can exist in human cells for a long time, it is generally difficult to distinguish current infection or previous infection by detecting antibodies. It can be judged based on the level of antibody titer and its kinetic changes.

2. Detection of antigens uses immunological methods to detect pathogens (tachyzoites or cysts) in host cells, metabolism or cleavage products (circulating antigens) in serum and body fluids. It is a reliable method for early diagnosis and confirmed diagnosis. Domestic and foreign scholars have established McAb-ELISA and sandwich ELISA methods of McAb and polyclonal antibodies to detect serum circulating antigens in acute patients. Their sensitivity is able to detect 0.4 μg/ml of antigens in serum.